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An integrated approach to assess impact of caecal microbiota on faecal metabolic phenotypes in the presence of Radix Scrophulariae involving 16S rRNA gene sequencing combined with UHPLC/TOF-MS-based faecal metabolomics was applied to Radix Scrophulariae-treated rats.

rsc advances

An integrated metabolomics and 16S rRNA gene sequencing approach exploring the molecular pathways and potential targets behind the effects of Radix Scrophulariae