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Biomacromolecule-based nanomaterials have attracted much attention due to their excellent function in sensing, catalysis, medicine, biology and recognition. In this work, a silane-coupling ionic liquid, 1-(3-trimethoxysilylpropyl)-3-methylimidazolium chloride ([TMIM]Cl), was synthesized and applied to prepare ionic liquid-functionalized nanoparticles (SiO 2 @IL) using surface grafting technology. By employing multiple non-covalent interactions, including electrostatic interactions, hydrogen bonding and π-π stacking, the obtained functional nanoparticles were able to bind bovine serum albumin (BSA) with strong binding affinity, which has been illustrated through experiments and theoretical calculations. Moreover, the stability of SiO 2 @IL further demonstrated that it is promising in applications for biomacromolecule immobilization.

Non-covalent loading of ionic liquid-functionalized nanoparticles for bovine serum albumin: experiments and theoretical analysis