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Silica nanoparticles were synthesized and chemically modified with iminodiacetic acid (IDA) and Ni 2+  ions surrounded by a bis-acrylamide polymeric shell to obtain a new core–shell immobilized metal affinity chromatography (IMAC) based material.

rsc advances

Bifunctional nickel–iminodiacetic acid-core–shell silica nanoparticles for the exclusion of high molecular weight proteins and purification of His-tagged recombinant proteins