English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Metallic nanoparticles were shown to affect F\"orster energy transfer betweenfluorophore pairs. However, to date, the net plasmonic effect on FRET is stillunder dispute, with experiments showing efficiency enhancement and reduction.This controversy is due to the challenges involved in the precise positioningof FRET pairs in the near field of a metallic nanostructure, as well as in theaccurate characterization of the plasmonic impact on the FRET mechanism. Here,we use the DNA origami technique to place a FRET pair 10 nm away from thesurface of gold nanoparticles with sizes ranging from 5 to 20 nm. In thisconfiguration, the fluorophores experience only moderate plasmonic quenching.We use the acceptor bleaching approach to extract the FRET rate constant andefficiency on immobilized single FRET pairs based solely on the donor lifetime.This technique does not require a posteriori correction factors neither apriori knowledge of the acceptor quantum yield, and importantly, it isperformed in a single spectral channel. Our results allow us to conclude that,despite the plasmon-assisted Purcell enhancement experienced by donor andacceptor partners, the gold nanoparticles in our samples have a negligibleeffect on the FRET rate, which in turns yields a reduction of the transferefficiency.

Plasmon-assisted F\"orster resonance energy transfer at the single-molecule level in the moderate quenching regime