English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Coconut cake albumin was hydrolysed by sequential digestion with alcalase, flavourzyme, pepsin and trypsin to purify bioactive peptides with ACE-inhibitory and antioxidant activities. Following fractionation with sequential ultrafiltration, Sephadex gel chromatography and RP-HPLC, three novel peptides KAQYPYV, KIIIYN and KILIYG were identified. KAQYPYV, KIIIYN and KILIYG provided an IC 50 value of 37.06 μM, 58.72 μM and 53.31 μM on ACE-inhibitory activity, respectively. For hydroxyl radical scavenging activity, KAQYPYV, KIIIYN and KILIYG demonstrated an IC 50 value of 70.84 μM, 77.62 μM and 95.23 μM, respectively. All the three peptides exhibited a mixed modality of noncompetitive and uncompetitive inhibition on ACE and KAQYPYV showed good stability against gastrointestinal enzymes digestion. Moreover, these peptides could effectively lower intracellular endothelin-1 content without significant cytotoxicity, and protected vascular endothelial cells from reactive oxygen species mediated damage. Furthermore, KAQYPYV, KIIIYN and KILIYG also demonstrated high ion chelating ability (62.13% ± 4.21%, 64.66% ± 5.51% and 69.82% ± 7.24% at 0.1 mg mL -1 , respectively) and considerable superoxide radical scavenging activity (39.30% ± 2.72%, 46.79% ± 1.70% and 51.16% ± 3.23% at 1.0 mg mL -1 , respectively). These results indicate that coconut cake albumin is a potential source of bioactive peptides possessing ACE-inhibitory and antioxidant activities.

ACE-inhibitory and antioxidant peptides from coconut cake albumin hydrolysates: purification, identification and synthesis