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Rhodamine B derivatives (RBP)-modified UCNPs (UCNPs@mSiO2–RBP) were developed as a fluorescent turn-off–on sensor based on FRET and IFE to detect Cu2+ and pyrophosphate (PPi) with a wide linear response range (0–10 μM for Cu2+ and 5–35 μM for PPi, much wider than that reported previously) and high sensibility (117 nM for Cu2+ and 70 nM for PPi). The MTT experiments and the bioimaging experiments show its promising prospect in tissue imaging.

rsc advances

Rhodamine B derivatives-modified upconversion nanoparticles as a fluorescent turn-off–on sensor for the highly sensitive detection of Cu<sup>2+</sup> and pyrophosphate

Rhodamine B derivatives-modified upconversion nanoparticles as a fluorescent turn-off–on sensor for the highly sensitive detection of Cu2+ and pyrophosphate

Rhodamine B derivatives-modified upconversion nanoparticles as a fluorescent turn-off–on sensor for the highly sensitive detection of Cu²⁺ and pyrophosphate