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Nano-liposomes were designed for the sustained release of water soluble compounds from C. sinensis CS1197 using a supercritical gas anti-solvent (SC-GAS) method at various pressures, temperatures and Tween 80 concentrations. The SC-GAS method was compared to the Bangham method of liposome production in terms of mean diameter, coefficient of uniformity ( C  u ), encapsulation efficiency, morphology, viscosity and actual energy required for liposome formation. Liposome production via the SC-GAS method under optimized formulation conditions, i.e. , 180 bar; 50 °C; 0.75% Tween 80; and a depressurization rate of 25 bar min -1 , yielded nano-liposomes exhibiting the lowest C  u value (1.10 ± 0.012) with a mean diameter of 0.072 ± 0.002 μm and better encapsulation efficiencies of 75.48 ± 2.5, 74.9 ± 2.1 and 70.23 ± 2.9% for adenosine, cordycepin and polysaccharides, respectively. Nano-liposomes were characterized using FTIR, XRD, DSC and TGA techniques. The stability indices and viscosities of the prepared liposome suspensions indicated good stability of up to 2 months and near-Newtonian behavior. The in vitro release of CS1197 water soluble compounds exhibited biphasic and sustained release patterns.

rsc advances

Nanocharacterization of liposomes for the encapsulation of water soluble compounds from<i>Cordyceps sinensis</i>CS1197 by a supercritical gas anti-solvent technique

Nanocharacterization of liposomes for the encapsulation of water soluble compounds fromCordyceps sinensisCS1197 by a supercritical gas anti-solvent technique