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Photocleavable liposomes were formed in situ through the coupling of an o -nitrobenzyl-containing azide tail precursor and an alkyne-functionalized lysolipid by the copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. Inclusion of the photolabile o -nitrobenzyl-structure enables control over the permeability and morphology of the liposomes. Photolysis of the o -nitrobenzyl group changes the molecular structure of the photolabile phospholipids, inducing phase transitions and permeability increases in the bilayer membrane, ultimately disrupting the liposome entity.

Liposomes formed from photo-cleavable phospholipids: in situ formation and photo-induced enhancement in permeability