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Herein, we report a highly sensitive colorimetric sensor for the detection of heparin based on its anti-aggregation effect of the PDDA–gold nanoparticle colloidal system. PDDA-induced non-crosslinking aggregation of gold nanoparticles is firstly investigated and the phenomenon of heparin mediated color recovery is then observed, which can be used to indicate the concentration of heparin. This method is proved to be highly sensitive and selective. Moreover, it has been successfully applied to determine human blood serum samples, the results of which demonstrate great potential practical utility with simple operations.

Colorimetric sensing strategy for heparin assay based on PDDA-induced aggregation of gold nanoparticles