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Gold nanoparticle based metal enhanced fluorescence applied to the Europium Nanoparticle Immunoassay (ENIA) improves the limit of detection by ten-fold from 1.8 pg mL −1 to 0.19 pg mL −1 .

Sub-picogram level sensitivity in HIV diagnostics achieved with the europium nanoparticle immunoassay through metal enhanced fluorescence