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We report an enzyme-activated fluorescence based probe (NHPO) for the selective detection of N-acetyl-β-d-glucosaminidase (NAG) activity in a drug-induced kidney injury mice model and NAG level in crude human urine. We used NHPO to explore the biological functions of NAG and provide a reference for the prognosis and prediction of proximal renal tubular dysfunction associated with various kidney diseases.

NAG-targeting fluorescence based probe for precision diagnosis of kidney injury