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An organic electrochemical transistor (OECT) based on poly(3,4-ethylenedioxythiophene):polystyrenesulfonate with porous anodic aluminum oxide (AAO) as a gate electrode was proposed for DNA sensing.The AAO substrate, which possesses a pore size of 200 nm, underwent gold nanofilm deposition by thethermal evaporation technique and is referred to as a nanoporous gold electrode in this work. Due to itshigh sensitivity, target DNA as low as 0.1 nM in concentration was achieved on an OECT device witha nanoporous gold gate electrode using a peptide nucleic acid probe. One-base and two-basemismatched DNA sequences could be discriminated. A sensing mechanism for the OECT device basedon the nanoporous gold electrode was proposed. The synergetic effect of capacitance with the surfacepotential on the nanoporous gold electrode enlarged the increase in gate potential, which resulted inhigh sensitivity for DNA sensing

A sensitive DNA sensor based on an organic electrochemical transistor using a peptide nucleic acid-modified nanoporous gold gate electrode