English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The Saccharomyces cerevisiae strain UV02_HG, which has faster growth and a higher ethanol yield in 40% (w/v) glucose culture medium than the wild type, is a mutant generated from the MF02 wild-type strain by UV irradiation. Transcriptome sequencing was performed to analyze the differential gene expression between the two strains. 1203 genes had significantly different expression levels in response to high sugar stress. Based on KEGG enrichment analysis, the identified genes were involved in pathways such as the ribosome, oxidative phosphorylation, protein processing, protein export, carbon metabolism, and the MAPK pathway. Real-time quantitative PCR (qPCR) was used to validate the reliability of the results obtained by RNA-Seq. The network-based gene expression Quantitative Trait Locus (eQTL) tool was used to interpret the driver mutations that elicited the adaptive phenotype. According to this network, we speculated that mutations in STE12 and TUP1 are the main drivers of the adaptive phenotype. Sixteen poorly understood genes whose expression was downregulated by STE12 were annotated by gene ontology, all of which are highly associated with the cell membrane.

rsc advances

Transcriptomic analysis and driver mutant prioritization for differentially expressed genes from a Saccharomyces cerevisiae strain with high glucose tolerance generated by UV irradiation