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A new method to recognize human transferrin (Tf) conformations was developed using a cyanine dye supramolecular assembly. We achieved detection of the open conformation of Tf (apo-Tf) in a sub-micromolar level against the closed one (holo-Tf). As a protein conformational probe, it's promising to monitor the transition between the two conformations of Tf.

Specific identification of human transferrin conformations using a cyanine dye supramolecular assembly