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We report a simple technique for the high throughput generation of tissue spheroids based on the encapsulation of dispersed adherent cells in particle-stabilised water-in-water emulsions. We demonstrate cell encapsulation and spheroid generation using fibroblast cells which can find applications in 3D culturing, tissue engineering, inks for bioprinting and cell therapy.

High throughput fabrication of cell spheroids by templating water-in-water Pickering emulsions