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In the present study, a novel fluorescence resonance energy transfer (FRET) strategy-based two-photon excited nanosensor via the assembly of two-photon dye-labeled peptides on the gold nanoparticle's surface has been developed and successfully applied in the caspase-3 imaging assay in living cells and ischemia-reperfusion surgery-treated rat liver tissue slices.

Peptide-fluorophore/AuNP conjugate-based two-photon excited fluorescent nanosensor for caspase-3 activity imaging assay in living cells and tissue