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Phosphatidylserine (PS) in the plasma membrane of single apoptotic cells was detected using luminol electrochemiluminescence for the first time. Membrane PS was converted by aqueous phospholipase D and L-amino acid oxidase to generate hydrogen peroxide, inducing electrochemically generated luminescence. The successful observation of PS in the cellular membrane provided a special strategy for the observation of cellular apoptosis of single cells.

Detection of phosphatidylserine in the plasma membrane of single apoptotic cells using electrochemiluminescence