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We have developed a microchip electrophoresis (MCE)-based fluorescence signal amplification strategy as a universal MCE method for the detection of trace biomolecules. This strategy exhibits high sensitivity and specificity for target molecules, and has been applied for the detection of interferon-gamma (IFN-γ) in human plasma with satisfactory results.

A microchip electrophoresis-based fluorescence signal amplification strategy for highly sensitive detection of biomolecules