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Pseudomonas aeruginosa biofilm was cultivated and characterized in a microfluidic "lab-on-a-chip" platform coupled with confocal Raman microscopy in a non-destructive manner. Biofilm formation could be quantified by this label-free platform and correlated well with confocal laser scanning microscopy. This Raman-microfluidic platform could also discriminate biofilms at different developmental stages.

chemical communications

An in situ Raman spectroscopy-based microfluidic “lab-on-a-chip” platform for non-destructive and continuous characterization of Pseudomonas aeruginosa biofilms