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Two second-generation quadruplex hammerhead ribozymes, whose activity enhances in response to K(+)via quadruplex formation of embedded r(GGA)3GG, were developed. Different strategies were applied to suppress basal activity when K(+) is absent. As a result, the activity enhancement upon the addition of K(+) has reached as high as 21-fold.

Boosting of activity enhancement of K+-responsive quadruplex hammerhead ribozyme

Boosting of activity enhancement of K⁺-responsive quadruplex hammerhead ribozyme