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Micro-Infrared Spectroscopy (mSR-FTIR) and X-ray diffraction (mSR-XRD) with synchrotron light, Gas\udChromatography/Mass Spectrometry (CG/MS), Optical Microscopy (OM) and Scanning Electron\udMicroscopy (SEM/EDS) were used to identify and obtain the distribution of complex mixtures of calcium\udsalts of low molecular weight organic acids (LMWOA) in micro-layered micro-samples. Filamentous fungi\udproduce LMWOA that can react with metal cations producing stable salts. These substances were found\udin the dark spots covering the surfaces of Saint Michael's Chapel wall paintings of the Royal Monastery of\udPedralbes in Barcelona linking them to old fungi activity. The presence of glycerol likewise related to the\udfungi activity is also identified in the layers.Postprint (author's final draft

Low molecular weight organic acid salts, markers of old fungi activity in wall paintings