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The availability of green and cheap technologies to recover polyhydroxyalkanoates (PHAs) from microbialbiomass is crucial for the development of a reliable and sustainable production chain. Here, two novelprotocols are proposed to extract PHAs from Cupriavidus necator. The first method is based on PHAextractionwith dimethyl carbonate (DMC), a green solvent that is completely biodegradable and lessharmful to humans and the environment than most solvents. The procedure can be applied directly toconcentrated microbial slurries or to dry biomass, affording very high polymer recovery (>85%) and excellentpurity (>95%). No degradation/decomposition of the polymer is observed in both cases. The secondprotocol uses fatty acid carboxylates as surfactants, which disrupt cell membranes, providing excellentpolymer recovery (>99%) and high purity (>90%). Ammonium laurate can be successfully used and easilyrecycled (98%) by lowering the pH through CO2 addition. Therefore, both protocols reported here areeffective and sustainable: the recovery and purity of the obtained PHAs are very high, the use of toxicchemicals is avoided, and the recycling of various solvents/surfactants used in the processes is optima

Dimethyl carbonate and switchable anionic surfactants: two effective tools for the extraction of polyhydroxyalkanoates from microbial biomass