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Fludioxonil is a fungicide with a singular mode of action that is widely employed in the treatment of fruit crops. A competitive enzyme-linked immunosorbent assay (cELISA) has been developed and validated for the determination of residues of this fungicide in fruit juices. A collection of monoclonal antibodies (mAbs) against the fungicide fludioxonil has been produced and characterized using direct and indirect cELISAs. The high affinity achieved (IC50lower than 0.5 μg L-1) considerably improved that obtained with previous polyclonal antibodies. The proposed cELISA was applied to the analysis of fludioxonil residues in apple juice and red grape must samples with a limit of quantification of 5 and 10 μg L-1, respectively. A validation of the method has been performed in terms of sensitivity, selectivity, trueness, precision, and robustness. Finally, blind-spiked samples were measured by the proposed cELISA and by a gas chromatography-mass spectrometry (GC-MS) method, with equivalent results by both methodologies.This work was supported by the Spanish Ministerio de Ciencia e Innovación (AGL2009-12940-C02-01-02/ALI) and cofinanced by FEDER funds. J.V.M. was hired by the Consejo Superior de Investigaciones Científicas (CSIC) under a Ramón y Cajal contract financed by Ministerio de Ciencia e Innovación and the European Social Fund. We acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). We thank Alba Jiménez-Cuesta for her technical assistance.Peer Reviewe

Development of a sensitive and specific enzyme-linked immunosorbent assay for the determination of fludioxonil residues in fruit juices