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Three step chemical tweaking of nonfluorescent parent GFP chromophore has yielded a three hundred times as bright coumarinic fluorophore. A fluorogenic compound has been prepared which after photo-uncaging yields the same highly bright fluorophore that could be used for live stem cell imaging.

rsc advances

Chemical tweaking of a non-fluorescent GFP chromophore to a highly fluorescent coumarinic fluorophore: application towards photo-uncaging and stem cell imaging