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The O2-binding carboxylate-bridged diiron site in DcrH-Hr was engineered in an effort to perform the H2O2-dependent oxidation of external substrates. A His residue was introduced near the diiron site in place of a conserved residue, Ile119. The I119H variant promotes the oxidation of guaiacol and 1,4-cyclohexadiene upon addition of H2O2.

H2O2-dependent substrate oxidation by an engineered diiron site in a bacterial hemerythrin

H₂O₂-dependent substrate oxidation by an engineered diiron site in a bacterial hemerythrin