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A gold nanoparticle-based competitive colorimetric assay can detect mismatched DNAs using MutS, a mismatch binding protein, and determine their relative binding affinities for this protein by a simple color change and the melting temperature of DNA-functionalized nanoparticle assemblies.

Detection of mismatched DNAs via the binding affinity of MutS using a gold nanoparticle-based competitive colorimetric method