English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

When producing recombinant proteins, the use of  Escherichia coli  strain BL21(DE3) in combination with the T7-based pET-expression system is often the method of choice. In a recent study we introduced a mechanistic model describing the correlation of the specific glucose uptake rate (q s,glu ) and the corresponding maximum specific lactose uptake rate (q s,lac,max ) for a pET-based  E. coli  BL21(DE3) strain producing a single chain variable fragment (scFv). We showed the effect of q s,lac,max  on productivity and product location underlining its importance for recombinant protein production. In the present study we investigated the mechanistic q s,glu /q s,lac,max  correlation for four pET-based  E. coli  BL21(DE3) strains producing different recombinant products and thereby proved the mechanistic model to be platform knowledge for  E. coli  BL21(DE3). However, we found that the model parameters strongly depended on the recombinant product. Driven by this observation we tested different dynamic bioprocess strategies to allow a faster investigation of this mechanistic correlation. In fact, we succeeded and propose an experimental strategy comprising only one batch cultivation, one fed-batch cultivation as well as one dynamic experiment, to reliably determine the mechanistic model for q s,glu /q s,lac,max  and get trustworthy model parameters for pET-based  E. coli  BL21(DE3) strains which are the basis for bioprocess development.

Mechanistic platform knowledge of concomitant sugar uptake in Escherichia coli BL21(DE3) strains