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Yeast is exposed to changing environmental conditions and must adapt its genetic program to provide a homeostatic intracellular environment. An important stress for yeast in the wild is high osmolarity. A key response to this stress is increased mRNA stability primarily by the inhibition of deadenylation. We previously demonstrated that mutations in decapping activators ( edc3∆ lsm4∆C ), which result in defects in P body assembly, can destabilize mRNA under unstressed conditions. We wished to examine whether mRNA would be destabilized in the  edc3∆ lsm4∆C  mutant as compared to the wild-type in response to osmotic stress, when P bodies are intense and numerous. Our results show that the  edc3∆ lsm4∆C  mutant limits the mRNA stability in response to osmotic stress, while the magnitude of stabilization was similar as compared to the wild-type. The reduced mRNA stability in the  edc3∆ lsm4∆C  mutant was correlated with a shorter  PGK1  poly(A) tail. Similarly, the  MFA2  mRNA was more rapidly deadenylated as well as significantly stabilized in the  ccr4∆  deadenylation mutant in the  edc3∆ lsm4∆C  background. These results suggest a role for these decapping factors in stabilizing mRNA and may implicate P bodies as sites of reduced mRNA degradation.

An mRNA decapping mutant deficient in P body assembly limits mRNA stabilization in response to osmotic stress