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A novel triterpenoid, holophyllane A ( 1 ), featuring a B-nor-3,4- seco -17,14- friedo -lanostane, along with its putative precursor, compound  2  were isolated from the methanol extract of the trunks of  Abies holophylla . The 2D structure and relative configuration of  1  were initially determined via analysis of 1D and 2D NMR spectroscopic data and the assignment was confirmed by quantum mechanics-based NMR chemical shift calculations. The absolute configuration was established by comparison of the experimental and simulated ECD data generated at different theory levels. Compounds  1  and  2  exhibited moderate to weak cytotoxicity and significant inhibitory activity against nitric oxide (NO) production.

Holophyllane A: A Triterpenoid Possessing an Unprecedented B-nor-3,4-seco-17,14-friedo-lanostane Architecture from Abies holophylla