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CdnL is an essential RNA polymerase (RNAP)-binding activator of rRNA transcription in mycobacteria and myxobacteria but reportedly not in  Bacillus . Whether its function and mode of action are conserved in other bacteria thus remains unclear. Because virtually all alphaproteobacteria have a CdnL homolog and none of these have been characterized, we studied the homolog (CdnL Cc ) of the model alphaproteobacterium  Caulobacter crescentus . We show that CdnL Cc  is not essential for viability but that its absence or depletion causes slow growth and cell filamentation. CdnL Cc  is degraded  in vivo  in a manner dependent on its C-terminus, yet excess CdnL Cc  resulting from its stabilization did not adversely affect growth. We find that CdnL Cc  interacts with itself and with the RNAP β subunit, and localizes to at least one rRNA promoter  in vivo , whose activity diminishes upon depletion of CdnL Cc . Interestingly, cells expressing CdnL Cc  mutants unable to interact with the RNAP were cold-sensitive, suggesting that CdnL Cc  interaction with RNAP is especially required at lower than standard growth temperatures in  C. crescentus . Our study indicates that despite limited sequence similarities and regulatory differences compared to its myco/myxobacterial homologs, CdnL Cc  may share similar biological functions, since it affects rRNA synthesis, probably by stabilizing open promoter-RNAP complexes.

Caulobacter crescentus CdnL is a non-essential RNA polymerase-binding protein whose depletion impairs normal growth and rRNA transcription