English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Polygalacturonase-inhibiting protein (PGIP), belonging to a group of plant defence proteins, specifically inhibits endopolygalacturonases secreted by pathogens. Herein, we showed that purified GhPGIP1 is a functional inhibitor of  Verticillium dahliae  and  Fusarium oxysporum  f. sp.  vasinfectum , the two fungal pathogens causing cotton wilt. Transcription of  GhPGIP1  was increased in cotton upon infection, wounding, and treatment with defence hormone and H 2 O 2 . Resistance by GhPGIP1 was examined by its virus-induced gene silencing in cotton and overexpression in  Arabidopsis . GhPGIP1-silenced cotton was highly susceptible to the infections. GhPGIP1 overexpression in transgenic  Arabidopsis  conferred resistance to the infection, accompanied by enhanced expression of pathogenesis-related proteins (PRs), isochorismate synthase 1 ( ICS1 ), enhanced disease susceptibility 1 ( EDS1 ), and phytoalexin-deficient 4 ( PAD4 ) genes. Transmission electron microscopy revealed cell wall alteration and cell disintegration in plants inoculated with polygalacturonase (PGs), implying its role in damaging the cell wall. Docking studies showed that GhPGIP1 interacted strongly with C-terminal of  V. dahliae  PG1 (VdPG1) beyond the active site but weakly interacted with C-terminal of  F. oxysporum  f. sp.  vasinfectum  (FovPG1). These findings will contribute towards the understanding of the roles of PGIPs and in screening potential combat proteins with novel recognition specificities against evolving pathogenic factors for countering pathogen invasion.

scientific reports

Molecular evidence for the involvement of a polygalacturonase-inhibiting protein, GhPGIP1, in enhanced resistance to Verticillium and Fusarium wilts in cotton