English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Wheat is recalcitrant to genetic transformation. A potential solution is to manipulate the expression of some host proteins involved in T-DNA integration process. VirE2 interacting protein 2 (VIP2) plays an important role in T-DNA transport and integration. In this study, a  TaVIP2  gene was cloned from common wheat. Southern blot and allele-specific polymerase chain reaction (AS-PCR) combined with an online chromosomal location software tool revealed that three  TaVIP2  genes were located on wheat chromosomes 1AL, 1BL, and 1DL. These three homoeoallelic  TaVIP2  genes all contained 13 exons and 12 introns, and their coding sequences were the same; there were a few single nucleotide polymorphisms (SNPs) among the three genes. The heterologous expression of the  TaVIP2  gene in tobacco led to enhancement of the  Agrobacterium -mediated transformation efficiency up to 2.5-fold. Transgenic tobacco plants expressing  TaVIP2  showed enhanced resistance to powdery mildew. Further quantitative real-time PCR (qRT-PCR) revealed that overexpression of  TaVIP2  in transgenic tobacco up-regulated the expression of an endogenous gene,  NtPR-1 , which likely contributed to powdery mildew resistance in transgenic tobacco. Our study indicates that the  TaVIP2  gene may be highly useful in efforts to improve  Agrobacterium -mediated transformation efficiency and to enhance powdery mildew resistance in wheat.

Cloning and characterization of TaVIP2 gene from Triticum aestivum and functional analysis in Nicotiana tabacum