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Post-transcriptional upregulation is an effective way to increase the expression of transgenes and thus maximize the yields of target chemicals from metabolically engineered organisms. Refractory elements in the 3′ untranslated region (UTR) that increase mRNA half-life might be available. In  Saccharomyces cerevisiae , several terminator regions have shown activity in increasing the production of proteins by upstream coding genes; among these terminators the  DIT1  terminator has the highest activity. Here, we found in  Saccharomyces cerevisiae  that two resident  trans -acting RNA-binding proteins (Nab6p and Pap1p) enhance the activity of the  DIT1  terminator through the  cis  element GUUCG/U within the 3′-UTR. These two RNA-binding proteins could upregulate a battery of cell-wall–related genes. Mutagenesis of the  DIT1  terminator improved its activity by a maximum of 500% of that of the standard  PGK1  terminator. Further understanding and improvement of this system will facilitate inexpensive and stable production of complicated organism-derived drugs worldwide.

Enhancement of protein production via the strong DIT1 terminator and two RNA-binding proteins in Saccharomyces cerevisiae