English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Peroxisomes are required for pathogenicity in many phytopathogenic fungi, but the relationships between fungal pathogenicity and peroxisomal function are not fully understood. Here, we report the identification of a T-DNA insertional mutant C445 of  Magnaporthe oryzae , which is defective in pathogenicity. Analysis of the mutation confirmed an insertion into the gene  MoPEX1 , which encodes a putative homologue to peroxin 1. Targeted gene deletion mutants of  MoPEX1  were nonpathogenic and were impaired in vegetative growth, conidiation, and appressorium formation.  ΔMopex1  mutants formed abnormal, less pigmented, and nonfunctional appressoria, but they were unable to penetrate plant cuticle. The  ΔMopex1  mutants were defective in the utilization of fatty acids (e.g., olive oil and Tween-20). Moreover, deletion of  MoPEX1  significantly impaired the mobilization and degradation of lipid droplets during appressorium development. Interestingly, deletion of  MoPEX1  blocked the import of peroxisomal matrix proteins. Analysis of an  M. oryzae  strain expressing GFP-MoPEX1 and RFP-PTS1 fusions revealed that MoPex1 localizes to peroxisomes. Yeast two hybrid experiments showed that MoPex1 physically interacts with MoPex6, a peroxisomal matrix protein important for fungal morphogenesis and pathogenicity. Taken together, we conclude that  MoPEX1  plays important roles in peroxisomal function and is required for infection-related morphogenesis and pathogenicity in  M. oryzae .

Identification and characterization of the peroxin 1 gene MoPEX1 required for infection-related morphogenesis and pathogenicity in Magnaporthe oryzae