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Conventional protein kinase Cs (cPKCs) are key signaling proteins for transducing intracellular Ca 2+  signals into downstream phosphorylation events. However, the lifetime of individual membrane-bound activated cPKCs is an order of magnitude shorter than the average time needed for target-protein phosphorylation. Here, we employed intermolecular Förster resonance energy transfer (FRET) in living cells combined with computational analysis to study the spatial organization of cPKCs bound to the plasma membrane. We discovered Ca 2+ -dependent cPKC nano-clusters that significantly extend cPKC’s plasma-membrane residence time. These protein patterns resulted from self-assembly mediated by Ca 2+ -binding C2-domains, which are widely used for membrane-targeting of Ca 2+ -sensing proteins. We also established clustering of other unrelated C2-domain containing proteins, suggesting that nano-cluster formation is a key step for efficient cellular Ca 2+ -signaling.

C2-domain mediated nano-cluster formation increases calcium signaling efficiency