English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Insects can rapidly adapt to environmental changes through physiological responses. The red flour beetle  Tribolium castaneum  is widely used as a model insect species. However, the stress–response system of this species remains unclear. Superoxide dismutase 2 (SOD2) is a crucial antioxidative enzyme that is found in mitochondria.  T. castaneum  SOD2 (TcSOD2) is composed of 215 amino acids, and has an iron/manganese superoxide dismutase domain. qRT-PCR experiments revealed that TcSOD2 was present through all developmental stages. To evaluate TcSOD2 function in  T. castaneum , we performed RNAi and also assessed the phenotype and antioxidative tolerance of the knockdown of  TcSOD2  by exposing larvae to paraquat. The administration of paraquat resulted in significantly higher 24-h mortality in  TcSOD2  knockdown larval groups than in the control groups. The  TcSOD2  knockdown adults moved significantly more slowly, had lower ATP content, and exhibited a different body color from the control groups. We found that  TcSOD2  dsRNA treatment in larvae resulted in increased expression of tyrosinase and laccase2 mRNA after 10 days. This is the first report showing that TcSOD2 has an antioxidative function and demonstrates that  T. castaneum  may use an alternative antioxidative system when the SOD2-based system fails.

scientific reports

Superoxide dismutase 2 knockdown leads to defects in locomotor activity, sensitivity to paraquat, and increased cuticle pigmentation in Tribolium castaneum