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Toxins are believed to play a crucial role in  Burkholderia pseudomallei  pathogenicity, however to date, only a few have been identified. The discovery of additional toxic molecules is limited by the lack of a sensitive indicator of  B. pseudomallei  toxicity. Previously, from a whole genome transcriptome analysis of  B. pseudomallei -infected  Caenorhabditis elegans , we noted significant overexpression of a number of worm genes encoding detoxification enzymes, indicating the host’s attempt to clear bacterial toxic molecules. One of these genes,  ugt–29 , a family member of UDP-glucuronosyltransferases, was the most robustly induced phase II detoxification gene. In this study, we show that strong induction of  ugt–29  is restricted to infections by the most virulent species among the pathogens tested. We also noted that  ugt–29  is activated upon disruption of host protein synthesis. Hence, we propose that UGT–29 could be a promising biosensor to detect  B. pseudomallei  toxins that compromise host protein synthesis. The identification of bactobolin, a polyketide-peptide hybrid molecule, as a toxic molecule of  B. pseudomallei  further verifies the utilization of this surveillance system to search for bacterial toxins. Hence, a  ugt–29  based reporter should be useful in screening for other molecules that inhibit host protein synthesis.

Detection of Burkholderia pseudomallei toxin-mediated inhibition of protein synthesis using a Caenorhabditis elegans ugt–29 biosensor