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We developed a novel approach to assess tumor vascularity using recombinant  Gaussia  luciferase (rGluc) protein and bioluminescence imaging. Upon intravenous injection of rGluc followed by its substrate coelenterazine, non-invasive visualization of tumor vascularity by bioluminescence imaging was possible. We applied this method for longitudinal monitoring of tumor vascularity in response to the anti-angiogenic drug tivozanib. This simple and sensitive method could be extended to image blood vessels/vasculature in many different fields.

Imaging Tumor Vascularity and Response to Anti-Angiogenic Therapy Using Gaussia Luciferase