English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Sterol glycosyltransferases ( SGT s) catalyse transfer of glycon moiety to sterols and their related compounds to produce diverse glyco-conjugates or steryl glycosides with different biological and pharmacological activities. Functional studies of  SGTs  from  Withania somnifera  indicated their role in abiotic stresses but details about role under biotic stress are still unknown. Here, we have elucidated the function of  SGTs  by silencing  SGTL1 ,  SGTL2  and  SGTL4  in  Withania somnifera . Down-regulation of  SGTs  by artificial miRNAs led to the enhanced accumulation of withanolide A, withaferin A, sitosterol, stigmasterol and decreased content of withanoside V in Virus Induced Gene Silencing (VIGS) lines. This was further correlated with increased expression of  WsHMGR, WsDXR, WsFPPS, WsCYP710A1, WsSTE1  and  WsDWF5  genes, involved in withanolide biosynthesis. These variations of withanolide concentrations in silenced lines resulted in pathogen susceptibility as compared to control plants. The infection of  Alternaria alternata  causes increased salicylic acid, callose deposition, superoxide dismutase and H 2 O 2  in aMIR-VIGS lines. The expression of biotic stress related genes, namely,  WsPR1, WsDFS, WsSPI  and  WsPR10  were also enhanced in aMIR-VIGS lines in time dependent manner. Taken together, our observations revealed that a positive feedback regulation of withanolide biosynthesis occurred by silencing of  SGTLs  which resulted in reduced biotic tolerance.

Silencing of sterol glycosyltransferases modulates the withanolide biosynthesis and leads to compromised basal immunity of Withania somnifera