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Zinc finger AN1-type domain 3 ( ZFAND3 ) is essential for spermatogenesis in mice. However, its function in teleosts remains unclear. In this study, we characterized the  ZFAND3  gene (termed as  OsZFAND3 ) in an important food fish, tilapia. The  OsZFAND3  cDNA sequence is 1,050 bp in length, containing an ORF of 615 bp, which encodes a putative peptide of 204 amino acid residues. Quantitative real-time PCR revealed that the  OsZFAND3  transcripts were exclusively expressed in the testis and ovary.  In situ  hybridization showed that the high expression of  OsZFAND3  transcripts was predominantly localized in the spermatocyte and spermatid. These results suggest that  OsZFAND3  is involved in male germ cell maturation. Three single nucleotide polymorphisms (SNPs) were detected in the introns of  OsZFAND3 . The  OsZFAND3  gene was mapped in the sex-determining locus on linkage group 1 (LG1). The three SNPs in the  OsZFAND3  gene were strictly associated with sex phenotype, suggesting that the  OsZFAND3  gene is tightly linked to the sex-determining locus. Our study provides new insights into the functions of the  OsZFAND3  gene in tilapia and a foundation for further detailed analysis of the  OsZFAND3  gene in sex determination and differentiation.

Charactering the ZFAND3 gene mapped in the sex-determining locus in hybrid tilapia (Oreochromis spp.)