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Rice false smut caused by  Ustilaginoidea virens  is one of the most important diseases of rice worldwide. Although its genome has been sequenced, to date there is no report on targeted gene deletion in  U. virens  and no molecular studies on genetic mechanisms regulating the infection processes of this destructive pathogen. In this study, we attempted to generate knockout mutants of the ortholog of yeast  HOG1  MAP kinase gene in  U. virens . One  Uvhog1  deletion mutant was identified after screening over 600 hygromycin-resistant transformants generated by  Agrobacterium tumefaciens  mediated transformation. The  Uvhog1  mutant was reduced in growth rate and conidiation but had increased sensitivities to SDS, Congo red, and hyperosmotic stress. Deletion of  UvHOG1  resulted in reduced expression of the stress response-related genes  UvATF1  and  UvSKN7 . In the  Uvhog1  mutant, NaCl treatment failed to stimulate the accumulation of sorbitol and glycerol. In addition, the  Uvhog1  mutant had reduced toxicity on shoot growth in rice seed germination assays. Overall, as the first report of targeted gene deletion mutant in  U. virens , our results showed that  UvHOG1  likely has conserved roles in regulating stress responses, hyphal growth, and possibly secondary metabolism.

UvHOG1 is important for hyphal growth and stress responses in the rice false smut fungus Ustilaginoidea virens