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A novel sandwich-type electrochemical immunosensor using the new amino group functionalized silicoaluminophosphates molecular sieves (NH 2 -SAPO-34) supported Pd/Co nanoparticles (NH 2 -SAPO-34-Pd/Co NPs) as labels for the detection of bladder cancer biomarker nuclear matrix protein-22 (NMP-22) was developed in this work. The reduced graphene oxide-NH (rGO-NH) with good conductivity and large surface area was used to immobilize primary antibody (Ab 1 ). Due to the excellent catalytic activity toward hydrogen peroxide, NH 2 -SAPO-34-Pd/Co NPs were used as labels and immobilized secondary antibody (Ab 2 ) through adsorption capacity of Pd/Co NPs to protein. The immunosensor displayed a wide linear range (0.001–20 ng/mL) and low detection limit (0.33 pg/mL). Good reproducibility and stability have showed satisfying results in the analysis of clinical urine samples. This novel and ultrasensitive immunosensor may have the potential application in the detection of different tumor markers.

Sensitive Electrochemical Immunosensor for Detection of Nuclear Matrix Protein-22 based on NH2-SAPO-34 Supported Pd/Co Nanoparticles