English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Heat shock response (HSR) generally plays a major role in sustaining protein homeostasis. In  Escherichia coli,  the activity and amount of the dedicated transcription factor σ 32  transiently increase upon heat shock. The initial induction is followed by chaperone-mediated negative feedback to inactivate and degrade σ 32 . Previous work reported that signal recognition particle (SRP)-dependent targeting of σ 32  to the membrane is essential for feedback control, though how SRP recognizes σ 32  remained unknown. Extensive photo- and disulfide cross-linking studies  in vivo  now reveal that the highly conserved regulatory region of σ 32  that lacks a consecutive hydrophobic stretch interacts with the signal peptide-binding site of Ffh (the protein subunit of SRP). Importantly, the σ 32 –Ffh interaction observed was significantly affected by mutations in this region that compromise the feedback regulation, but not by deleting the DnaK/DnaJ chaperones. Homeostatic regulation of HSR thus requires a novel type of SRP recognition mechanism.

A Novel SRP Recognition Sequence in the Homeostatic Control Region of Heat Shock Transcription Factor σ32