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Herein, a novel and ultrasensitive label-free electrochemical immunosensor was proposed for quantitative detection of human Immunoglobulin G (IgG). The amino functionalized magnetic graphenes nanocomposites (NH 2  - GS - Fe 3 O 4 ) were prepared to bond gold and silver core-shell nanoparticles (Au@Ag NPs) by constructing stable Au - N and Ag - N bond between Au@Ag NPs and -NH 2 . Subsequently, the Au@Ag/GS - Fe 3 O 4  was applied to absorb cadmium ion (Cd 2+ ) due to the large surface area, high conductivity and exceptional adsorption capability. The functional nanocomposites of gold and silver core-shell magnetic graphene loaded with cadmium ion (Au@Ag/GS - Fe 3 O 4 /Cd 2+ ) can not only increase the electrocatalytic activity towards hydrogen peroxide (H 2 O 2 ) but also improve the effective immobilization of antibodies because of synergistic effect presented in Au@Ag/GS - Fe 3 O 4 /Cd 2+ , which greatly extended the scope of detection. Under the optimal conditions, the proposed immunosensor was used for the detection of IgG with good linear relation in the range from 5 fg/mL to 50 ng/mL with a low detection limit of 2 fg/mL (S/N = 3). Furthermore, the proposed immunosensor showed high sensitivity, special selectivity and long-term stability, which had promising application in bioassay analysis.

scientific reports

An ultrasensitive label-free electrochemical immunosensor based on signal amplification strategy of multifunctional magnetic graphene loaded with cadmium ions