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Currently, only few glufosinate-resistant genes are available for commercial application. Thus, developing novel glufosinate-resistant genes with commercial feasibility is extremely important and urgent for agricultural production. In this study, we transferred a newly isolated  RePAT  gene into a  japonica  rice variety Zhonghua11, resulting in a large number of independent T 0  transgenic plants, most of which grew normally under high-concentration glufosinate treatment. Four transgenic plants with one intact  RePAT  expression cassette integrated into the intergenic region were selected. Agronomic performances of their T 2  progenies were investigated, and the results suggested that the expression of  RePAT  had no adverse effect on the agronomic performance. Definite glufosinate resistance of the selected transgenic plants was further confirmed to be related to the expression of  RePAT  by assay on the medium and qRT-PCR. The inheritance and expression of  RePAT  in two transgenic plants were confirmed to be stable. Finally, the two-year field assay of glufosinate resistance suggested that the agronomic performance of the transgenic plant (PAT11) was not affected by high dosage of glufosinate (5000 g/ha). Collectively, our study proves the high resistance of a novel gene  RePAT  to glufosinate and provides a glufosiante-resistant rice variety with agricultural application potential.

Application of a novel phosphinothricin N-acetyltransferase (RePAT) gene in developing glufosinate-resistant rice