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With its traditional use in relieving insomnia and anxiety, our previous study has identified onjisaponin B from  Radix Polygalae  ( RP ), as a novel autophagic enhancer with potential neuroprotective effects. In current study, we have further identified a novel active fraction from  RP , contains 17 major triterpenoid saponins including the onjisaponin B, by the combinational use of cell membrane chromatography (CMC) and ultra-performance liquid chromatography coupled to (quadrupole) time-of-flight mass spectrometry {UHPLC-(Q)TOF-MS}. By exhibiting more potent autophagic effect in cells, the active fraction enhances the clearance of mutant huntingtin, and reduces protein level and aggregation of α-synuclein in a higher extent when compared with onjisaponin B. Here, we have reported for the first time the new application of cell-based CMC and UHPLC-(Q)TOF-MS analysis in identifying new autophagy inducers with neuroprotective effects from Chinese medicinal herb. This result has provided novel insights into the possible pharmacological actions of the active components present in the newly identified active fraction of  RP , which may help to improve the efficacy of the traditional way of prescribing  RP , and also provide new standard for the quality control of decoction of  RP  or its medicinal products in the future.

scientific reports

Identification of novel autophagic Radix Polygalae fraction by cell membrane chromatography and UHPLC-(Q)TOF-MS for degradation of neurodegenerative disease proteins