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Insect pests cause serious crop damage and develop high-level resistance to chemical insecticides and  Bacillus thuringiensis  (Bt) insecticidal Cry toxins. A new promising approach for controlling them and overcoming this resistance is RNA interference (RNAi). The RNAi-based insect control strategy depends on the selection of suitable target genes. In this study, we cloned and characterized a novel ABC transporter gene  PxABCH1  in diamondback moth,  Plutella xylostella  (L.). Phylogenetic analysis showed that  PxABCH1  is closely related to ABCA and ABCG subfamily members. Spatial-temporal expression detection revealed that  PxABCH1  was expressed in all tissues and developmental stages, and highest expressed in head and male adult. Midgut sequence variation and expression analyses of  PxABCH1  in all the susceptible and Bt-resistant  P. xylostella  strains and the functional analysis by sublethal RNAi demonstrated that Cry1Ac resistance was independent of this gene. Silencing of  PxABCH1  by a relatively high dose of dsRNA dramatically reduced its expression and resulted in larval and pupal lethal phenotypes in both susceptible and Cry1Ac-resistant  P. xylostella  strains. To our knowledge, this study provides the first insight into ABCH1 in lepidopterans and reveals it as an excellent target for RNAi-based insect pest control and resistance management.

The novel ABC transporter ABCH1 is a potential target for RNAi-based insect pest control and resistance management