English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Bio-based production of chemicals from renewable resources is becoming increasingly important for sustainable chemical industry. In this study,  Escherichia coli  was metabolically engineered to produce 1,3-diaminopropane (1,3-DAP), a monomer for engineering plastics. Comparing heterologous C 4  and C 5  pathways for 1,3-DAP production by genome-scale  in silico  flux analysis revealed that the C 4  pathway employing  Acinetobacter baumannii dat  and  ddc  genes, encoding 2-ketoglutarate 4-aminotransferase and L-2,4-diaminobutanoate decarboxylase, respectively, was the more efficient pathway. In a strain that has feedback resistant aspartokinases, the  ppc  and  aspC  genes were overexpressed to increase flux towards 1,3-DAP synthesis. Also, studies on 128 synthetic small RNAs applied in gene knock-down revealed that knocking out  pfkA  increases 1,3-DAP production. Overexpression of  ppc  and  aspC  genes in the  pfkA  deleted strain resulted in production titers of 1.39 and 1.35 g l −1  of 1,3-DAP, respectively. Fed-batch fermentation of the final engineered  E. coli  strain allowed production of 13 g l −1  of 1,3-DAP in a glucose minimal medium.

Metabolic engineering of Escherichia coli for the production of 1,3-diaminopropane, a three carbon diamine