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In previous studies we have demonstrated that chlorophyllide  a  oxidoreductases (CORs) from bacteriochlorophyll (BChl)  a -producing  Rhodobacter  species and BChl  b -producing  Blastochloris viridis  show distinct substrate recognition and different catalytic hydrogenation reactions, and that these two types of CORs therefore cause committed steps for BChls  a  and  b  biosynthesis. In this study, COR genes from  B. viridis  were incorporated and overexpressed in a series of  Rhodobacter sphaeroides  mutants. We found that the following two factors are essential in making  R. sphaeroides  produce BChl  b : the loss of functions of both intrinsic COR and 8-vinyl reductase (BciA) in the host  R. sphaeroides  strain; and expression of the BchYZ catalytic components of COR from  B. viridis , not the complete set of COR (BchXYZ), in the host strain. In addition, we incorporated  bchYZ  of  B. viridis  into the  R. sphaeroides  mutant lacking BchJ and BciA, resulting in the strain accumulating both BChl  a  and BChl  b . This is the first example of an anoxygenic photosynthetic bacterium producing BChls  a  and  b  together. The results suggest that BchJ enhances activity of the intrinsic COR. The physiological significance of BchJ in pigment biosynthetic pathways will be discussed.

scientific reports

Rhodobacter sphaeroides mutants overexpressing chlorophyllide a oxidoreductase of Blastochloris viridis elucidate functions of enzymes in late bacteriochlorophyll biosynthetic pathways