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Autosomal Dominant Polycystic Kidney Disease (ADPKD) is a genetic disorder caused by loss-of-function mutations in  PKD1  or  PKD2 . Increased glycolysis is a prominent feature of the disease, but how it impacts on other metabolic pathways is unknown. Here, we present an analysis of mouse  Pkd1  mutant cells and kidneys to investigate the metabolic reprogramming of this pathology. We show that loss of  Pkd1  leads to profound metabolic changes that affect glycolysis, mitochondrial metabolism, and fatty acid synthesis (FAS). We find that  Pkd1 -mutant cells preferentially use glutamine to fuel the TCA cycle and to sustain FAS. Interfering with either glutamine uptake or FAS retards cell growth and survival. We also find that glutamine is diverted to asparagine via asparagine synthetase (ASNS). Transcriptional profiling of  PKD1 -mutant human kidneys confirmed these alterations. We find that silencing of  Asns  is lethal in  Pkd1 -mutant cells when combined with glucose deprivation, suggesting therapeutic approaches for ADPKD.

Dissection of metabolic reprogramming in polycystic kidney disease reveals coordinated rewiring of bioenergetic pathways